| Platinum | Final Form | Collect all other trophies for Jamestown+. | 1.1% Ultra Rare | 378 pts |
| Gold | Bullet Heaven | Survive the Gauntlet on Divine difficulty or higher. | 1.2% Ultra Rare | 179 pts |
| Silver | Centurion Card | Purchase all items from the Shoppe. | 1.3% Ultra Rare | 128 pts |
| Gold | No Mountain High Enough | Complete the 'Partition Rings' challenge level. | 1.6% Ultra Rare | 151 pts |
| Silver | No Valley Low Enough | Complete the 'Luge' challenge level. | 1.7% Ultra Rare | 109 pts |
| Gold | Impossible Combo | Score 500,000 points with a single Vaunt combo. | 2.1% Ultra Rare | 128 pts |
| Gold | Judgement Day | Complete any mission on Judgement difficulty. | 2.2% Ultra Rare | 125 pts |
| Gold | Veni, Vidi, Vici | Survive the Super Gauntlet. | 6.3% Very Rare | 66 pts |
| Silver | The Great Revival | Revive 3 teammates at the same time with a single revive token. | 6.5% Very Rare | 49 pts |
| Silver | Much Vaunted | Score 250,000 points with a single Vaunt combo. | 8.1% Very Rare | 43 pts |
| Gold | P(e)acemaker | Pacify the Heart of Mars. | 15.2% Rare | 39 pts |
| Silver | No Miss | Complete any level without dying. | 17.9% Rare | 27 pts |
| Silver | Gauntlet Runner | Survive the Gauntlet on any difficulty. | 18.7% Rare | 26 pts |
| Silver | CONQUISTADO-OR! | Defeat the Conquistador. | 21.2% Uncommon | 24 pts |
| Silver | Rope's End | Defeat the Pirate Queen. | 22.2% Uncommon | 23 pts |
| Bronze | A New Challenger! | Complete any of the challenge levels. | 30.4% Uncommon | 13 pts |
| Silver | Full Marks | Complete any level with a 5-star rating. | 33.7% Uncommon | 18 pts |
| Silver | Vauntless | Complete any level without using Vaunt. | 39.6% Uncommon | 17 pts |
| Silver | Final Destination | Defeat the Last Express. | 43.3% Common | 16 pts |
| Bronze | Long Live the Queen | Defeat the Matriarch. | 47.9% Common | 10 pts |
| Bronze | Jailbreak | Defeat the Dread Sentry. | 64.8% Common | 8 pts |
| Bronze | Ladykiller | Defeat the Lady of the Lake. | 70.2% Common | 8 pts |
| Bronze | Welcome Home... | Locate the Lost Colony of Roanoke. | 77.2% Common | 7 pts |
| Bronze | Tyrannicide | Defeat the Traitor Prince. | 85.3% Common | 7 pts |